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反相色譜柱壽命影響因素及延長使用壽命的方法
2024-5-15
反相色譜柱是液相色譜中常用的一種色譜柱類型,用于分離非極性或弱極性化合物。反相色譜柱的壽命是影響色譜分析效果和結果準確性的關鍵因素之一。在實際使用過程中,它的壽命受到多種因素的影響,包括樣品性質、流動相、操作條件等。本文將探討反相色譜柱壽命的影響因素以及延長使用壽命的方法。首先,樣品性質是影響壽命的重要因素之一。一些樣品可能具有蛋白質結合、化學活性或高濃度等特性,這些特性會導致表面的污染或損傷,從而縮短柱的壽命。因此,在選擇時,需要根據樣品性質選擇適合的柱材和填料,以減少對柱...
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Kromasil液相色譜柱167|中國藥典甲鈷胺
案例說明按照2020版中國藥典二部甲鈷胺有關物質測定分析方法。要求以十八烷基硅烷鍵合硅膠為填充劑,系統適用性溶液色譜圖中,甲鈷胺峰的保留時間約為13分鐘,甲鈷胺峰與相對保留時間約為1.16的雜質峰之間的分離度應大于3.0。使用Kromasil100-5-C18色譜柱進行了甲鈷胺應用方法實驗。甲鈷胺保留時間為13.812,甲鈷胺峰與雜質分離度約3.30,符合實驗要求,適用于中國藥典甲鈷胺項目的測定。分析條件色譜柱:Kromasil100-5-C18,4.6x250mm(訂貨號:...
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提高C18色譜柱分離效率的技巧
C18色譜柱是高效液相色譜中常用的反相色譜柱之一,廣泛應用于藥物分析、環境檢測、食品分析等領域。然而,在實際應用中,色譜峰拖尾、分離度不足、柱效下降等問題常常影響分析結果。通過優化這些參數,可以有效改善峰形、提高分離度并延長色譜柱壽命。定期維護和正確使用色譜柱是保證分析結果準確性和重現性的關鍵。1.選擇合適的流動相流動相的組成和pH值對C18色譜柱的分離效率有重要影響。(1)有機相與水相的比例-在反相色譜中,通常使用甲醇或乙腈作為有機相,水作為極性相。調整兩者的比例可以改變保...
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Kromasil液相色譜柱166|中國藥典鹽酸多西環素
案例說明按照2020版中國藥典二部鹽酸多西環素有關物質測定分析方法。要求以十八烷基硅烷鍵合硅膠為填充劑(pH值適用范圍應大于9),系統適用性溶液色譜圖中,多西環素峰與β-多西環素峰間的分離度應大于4.0。多西環素峰與雜質F峰(相對保留時間約為1.1)間的分離度應符合要求。使用KromasilEternityXT-5-C18色譜柱進行了鹽酸多西環素應用方法實驗。多西環素峰與β-多西環素峰間的分離度為8.72,大于4.0。多西環素峰與雜質F峰(相對保留時間約為1.128)間的分離...
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生物鬼峰捕集柱的質量標準
生物鬼峰捕集柱(Bio-GhostPeakCollectionColumn)是一種用于分析氣體或液體樣品中的痕量污染物、揮發性有機化合物(VOCs)以及環境監測中常見的氣體污染物的設備。其質量標準通常涉及材料選擇、制造精度、性能要求等多個方面。以下是一些常見的質量標準內容:1.材料與構造標準高純度材料:捕集柱的內部應采用高純度的吸附材料,如活性炭、分子篩、硅膠、樹脂等,這些材料具有高吸附性和較低的背景信號,以確保捕集柱對目標污染物的高效吸附和檢測。無污染性:所有接觸樣品的材料...
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濱松氘燈在紫外可見光譜分析中的優勢
濱松氘燈在紫外可見光譜分析中的優勢不容忽視,其寬廣的光譜覆蓋范圍、穩定的光輸出、長壽命和低維護需求、優良的抗干擾能力、兼容性與多樣化應用以及高性價比,使其成為了紫外可見光譜分析中的理想光源。1.寬廣的光譜覆蓋范圍一個顯著優勢是其寬廣的光譜輸出范圍。氘燈能夠發射從160nm到400nm的紫外光,這一波段是紫外可見光譜分析中最為重要的部分。對于需要分析紫外吸收的樣品,尤其是蛋白質、核酸等生物大分子,氘燈提供了理想的光源。此外,濱松氘燈的輸出穩定性也保證了在不同實驗條件下都能獲得高...
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如何使用鬼峰捕集柱實現高效的環境監測
鬼峰捕集柱作為高效的前處理技術,在環境監測中具有重要價值。通過合理選擇吸附材料、優化操作參數并結合先進的分析方法,可顯著提升環境樣品的檢測精度和效率。1.工作原理鬼峰通常由色譜系統中的污染物(如柱流失、載氣雜質或進樣口殘留物)引起,這些干擾峰會影響目標化合物的定性和定量分析。鬼峰捕集柱的作用是通過吸附或化學惰性材料截留這些干擾物,從而減少或消除鬼峰的產生。1.1捕集機制-吸附作用:捕集柱通常填充活性炭、分子篩或Tenax等吸附劑,可選擇性吸附揮發性有機物(VOCs)或半揮發性...
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2025-07-21
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